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avidin peroxidase  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin peroxidase
    Avidin Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/A-2004-5/Avidin+D%2C+Horseradish+Peroxidase+(Av-HRP)%2C+Concentrate%2C+for+IHC/bio_rxiv__64898__2026__04__23__720463-60-13-15
    Average 93 stars, based on 297 article reviews
    avidin peroxidase - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: CTLA4-Ig reduces proliferation and inflammatory gene expression in muscle fibroblasts, corresponding to less fibrosis and inflammation in mdx muscular dystrophy.
    Article Snippet: After a series of three, 10-minute washes in PBS, the tissues were 219 probed with species-specific, biotin-conjugated secondary antibodies (Vector Laboratories) diluted in 220 PBS to 1:200 for 30 minutes at room temperature (horse anti-goat IgG, Cat# BA-9500, 221 RRID:AB_2336123; rabbit anti-rat IgG, Cat# BA-4001, RRID:AB_10015300; horse anti-rabbit IgG, 222 Cat# BA-1100, RRID:AB_2336201; goat anti-hamster IgG, Cat# BA-9100, RRID:AB_2336137). .. 223 Washes were repeated and sections were incubated with avidin D-conjugated HRP (1:1000 in PBS, 224 30 minutes at room temperature, Vector Laboratories Cat# A-2004, RRID:AB_2336507). .. The 225 peroxidase substrate, AEC (3-amino-9-ethylcarbazole), was applied following the manufacturer’s 226 protocol (Vector Laboratories Cat# SK-4200, RRID:AB_2336076) and staining was visualized as a red 227 Downloaded from journals.physiology.org/journal/ajpcell (2401:4900:1CE3:2A95:9498:68CE:6ED0:8BFE) on March 21, 2026.

    Article Title: Delineating the effects of prenatal oxycodone exposure and melatonin treatment on placental and fetal outcomes in pregnant rats
    Article Snippet: Following overnight incubation, sections were washed in 1X TBST (0.6% Tween-20) and incubated for one hour with appropriate secondary antibodies (goat anti-mouse; Vector Lab #BA-9200 or goat anti-rabbit; Vector Lab #BA-1000). .. After three additional washes with 1X TBST (0.6% Tween-20), sections were incubated with avidin-peroxidase (1:200; Vector Laboratories #A-2004) for 30 minutes. .. Signal was developed using 3,3’-diaminobenzidine (Sigma-Aldrich #D4293) for 1 to 2 minutes, followed by rinsing in water, counterstaining with hematoxylin (Stat Lab #95-1), dehydration, and mounted using CoverSafe mounting medium (Stat Lab #MMC0226).

    Avidin-Biotin Assay:

    Article Title: CTLA4-Ig reduces proliferation and inflammatory gene expression in muscle fibroblasts, corresponding to less fibrosis and inflammation in mdx muscular dystrophy.
    Article Snippet: After a series of three, 10-minute washes in PBS, the tissues were 219 probed with species-specific, biotin-conjugated secondary antibodies (Vector Laboratories) diluted in 220 PBS to 1:200 for 30 minutes at room temperature (horse anti-goat IgG, Cat# BA-9500, 221 RRID:AB_2336123; rabbit anti-rat IgG, Cat# BA-4001, RRID:AB_10015300; horse anti-rabbit IgG, 222 Cat# BA-1100, RRID:AB_2336201; goat anti-hamster IgG, Cat# BA-9100, RRID:AB_2336137). .. 223 Washes were repeated and sections were incubated with avidin D-conjugated HRP (1:1000 in PBS, 224 30 minutes at room temperature, Vector Laboratories Cat# A-2004, RRID:AB_2336507). .. The 225 peroxidase substrate, AEC (3-amino-9-ethylcarbazole), was applied following the manufacturer’s 226 protocol (Vector Laboratories Cat# SK-4200, RRID:AB_2336076) and staining was visualized as a red 227 Downloaded from journals.physiology.org/journal/ajpcell (2401:4900:1CE3:2A95:9498:68CE:6ED0:8BFE) on March 21, 2026.

    Article Title: Influenza vaccine based on AS03-adjuvanted chimeric HA induces long-lived stalk-specific plasma cells in bone marrow and lymph nodes of nonhuman primates
    Article Snippet: .. Then, solution containing 1% FBS and HRP avidin D (1:5,000 dilution; A-2004, Vector Laboratories) was added to the wells for 2 h at RT. ..

    Article Title: Chimeric hemagglutinin-based universal influenza mRNA vaccine induces protective immunity and bone marrow plasma cells in rhesus macaques
    Article Snippet: Biotin-conjugated anti-monkey IgG antibody , Rockland , 617-106-012; RRID:AB_218723. .. Horseradish peroxidase (HRP) Avidin D , Vector Laboratories , A-2004; RRID:AB_2336507. .. Anti-monkey IgG/IgA/IgM antibody-heavy and light chain , Rockland , 617-101-130; RRID:AB_218708.

    Article Title: Delineating the effects of prenatal oxycodone exposure and melatonin treatment on placental and fetal outcomes in pregnant rats
    Article Snippet: Following overnight incubation, sections were washed in 1X TBST (0.6% Tween-20) and incubated for one hour with appropriate secondary antibodies (goat anti-mouse; Vector Lab #BA-9200 or goat anti-rabbit; Vector Lab #BA-1000). .. After three additional washes with 1X TBST (0.6% Tween-20), sections were incubated with avidin-peroxidase (1:200; Vector Laboratories #A-2004) for 30 minutes. .. Signal was developed using 3,3’-diaminobenzidine (Sigma-Aldrich #D4293) for 1 to 2 minutes, followed by rinsing in water, counterstaining with hematoxylin (Stat Lab #95-1), dehydration, and mounted using CoverSafe mounting medium (Stat Lab #MMC0226).

    Article Title: Early life ACE2 inhibition leads to long term anxiety-like behaviour in rats by disrupting the amygdala.
    Article Snippet: For DCX and parvalbumin, sections were incubated in primary antibody (goat anti-DCX: 1:500, 16–18 hr at 4 ◦C, Santa Cruz Biotechnology Inc., Dallas, TA, USA; or mouse anti- parvalbumin: 1:1000, 16–18 hr at 4 ◦C, Swant AG, Switzerland) followed by secondary antibody (biotinylated anti-goat: 1:500, Vector Laboratories, Newark, CA, USA; or biotinylated anti-mouse: 1:500, Vector Laboratories) for 2 hr at room temperature. .. We then used an avidin–biotin horseradish peroxidase (HRP) complex (ABC, 45 min, Vector Elite kit, Vector Laboratories) followed by diaminobenzidine with 0.0015 % H2O2 to visualize the HRP activity. ..

    Article Title: Adult telomerase positive stem cells: Effects of biological agents and characterization criteria on genomically-labeled clones and unlabeled clones of adult cells
    Article Snippet: • Biotin, goat anti-mouse IgG, #OB1126-21, Fisher • Biotin, goat anti-mouse IgG (fab specific), #B-7151, Sigma • Biotin, goat anti-rabbit IgG, #OB1316-21, Sigma • Biotin-SP, goat anti-mouse IgG, #JGM-065003, Accurate • Biotin-SP, goat anti-mouse fab-specific, #JGM-066003, Accurate • Biotinylated affinity purified, rat adsorbed anti-mouse IgG (H + L) (BA-2001, Vector Laboratories) • Goat anti-rat osteocalcin, #BT-413, Biomedical Technology .. • Avidin-peroxidase, #JSA-030083, Accurate • Horseradish peroxidase conjugated Avidin-D, #A-2004, Vector • Monoclonal anti-goat IgG clone GT-34 biotin, #B-3148, Sigma • Peroxidase Standard PK-4000 Vastatin ABC Reagent Kit, Vector Laboratories • Streptavidin-peroxidase, #JSA-030084, Accurate .. • ABTS kit, #506201, Kirkegaard and Perry • 4-Cloro-1-napthol, #C-8890, Sigma • 3,5-Diaminobenzoic acid dihydrochloride, #11-383-2, Aldrich • Vastatin ABC Reagent Kit, Vector Laboratories Inc., Burlingame, CA

    Article Title: Neuroinflammation does not exacerbate post-traumatic stress-disorder (PTSD)-like brain measures or behaviour after a traumatic event in male rats.
    Article Snippet: For DCX immunolabeling, hippocampal sections were incubated in primary antibody (goat anti-DCX: 1:500, 16–18 hr at 4 ◦C, Santa Cruz Biotechnology Inc., Dallas, TA, USA) followed by incubation with secondary antibody (biotinylated anti-goat: 1:500, Vector Laboratories, Newark, CA, USA) for 2 hr at room temperature. .. We then used an avidin–biotin horseradish peroxidase (HRP) complex (ABC, 45 min, Vector Elite kit, Vector Laboratories) followed by diaminobenzidine with 0.0015 % H2O2 to visualize the HRP activity. ..

    Activity Assay:

    Article Title: Early life ACE2 inhibition leads to long term anxiety-like behaviour in rats by disrupting the amygdala.
    Article Snippet: For DCX and parvalbumin, sections were incubated in primary antibody (goat anti-DCX: 1:500, 16–18 hr at 4 ◦C, Santa Cruz Biotechnology Inc., Dallas, TA, USA; or mouse anti- parvalbumin: 1:1000, 16–18 hr at 4 ◦C, Swant AG, Switzerland) followed by secondary antibody (biotinylated anti-goat: 1:500, Vector Laboratories, Newark, CA, USA; or biotinylated anti-mouse: 1:500, Vector Laboratories) for 2 hr at room temperature. .. We then used an avidin–biotin horseradish peroxidase (HRP) complex (ABC, 45 min, Vector Elite kit, Vector Laboratories) followed by diaminobenzidine with 0.0015 % H2O2 to visualize the HRP activity. ..

    Article Title: Neuroinflammation does not exacerbate post-traumatic stress-disorder (PTSD)-like brain measures or behaviour after a traumatic event in male rats.
    Article Snippet: For DCX immunolabeling, hippocampal sections were incubated in primary antibody (goat anti-DCX: 1:500, 16–18 hr at 4 ◦C, Santa Cruz Biotechnology Inc., Dallas, TA, USA) followed by incubation with secondary antibody (biotinylated anti-goat: 1:500, Vector Laboratories, Newark, CA, USA) for 2 hr at room temperature. .. We then used an avidin–biotin horseradish peroxidase (HRP) complex (ABC, 45 min, Vector Elite kit, Vector Laboratories) followed by diaminobenzidine with 0.0015 % H2O2 to visualize the HRP activity. ..



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